These experiment were conducted to study the effects of various vitamins and amino acids upon the growth of Saccharomyces rauxii T9 and the results obtained were as follows.
(1) As a growth factor, inositol, riboflavin, niacin and para amino benzoic acid were not required.
(2) Though biotin, thiamin and Ca-pantothenate were adaptably required, vitamins required in absolute were not existed.
(3) The growth rate of Saccharomyces rouxii T9 in the deficient group of biotin, thiamin, pyridoxine, riboflavin and Ca-pantothenate were more decreased on the media containing 26% of NaCl than containing none of NaCl in the earlystage of cultivation, while the growth rate showed the opposition tendency in the latter term of cultivation.
(4) As an amino acid, methionine, tryptophan, serine, threonine, cystine, glycine, leucine and valine showed systematically the effects of addition in the compound media containing (NH₄)₂SO₄ as a nitrogen sources.
(5) The growth rate of Saccharomyces rouxii T9 in the addition group of histidine, lycine, arginine, aspartic acid, proline and tyrosine were somewhat decreased on the media containing higher concentration of NaCl comparison with none NaCl media in the early stage of cultivation, showing the similar or increasing tendency in the latter term of cultivation.
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